An Electron Microscope Study of Mouse Mast Cells Arising in Vivo and in Vitro
نویسنده
چکیده
Selective differentiation of cells morphologically identical with tissue mast cells at the light microscope level has been described by Ginsburg and Sachs (5) and Ginsburg and Lagunoff (4) in a mouse tissue culture system composed of a C3H feeder layer overplated with horse serum-sensitized C57BI lymphoid cells . Histochemical evaluation indicates that the granules of the tissue culturederived mast cells, like the granules of mast cells from normal mouse connective tissue, contain strongly acidic mucopolysaccharides and a chymotrypsin-like enzyme (4), further suggesting a homology between the tissue culture-derived mast cells and native mast cells . In this report, the degree of homology between mast cells arising in
منابع مشابه
Morphology and Ultrastructure of Mouse Polarized Endometrial Epithelial Cell Monolyer in Vitro
Purpose: The objective for this study is to investigate the morphology and ultrastructure of mouse endometrial epithelial cell monolayer cultured on matrigel in dual-chambered system as an in vitro mouse endometrial epithelial cell culture model that mimics structural and functional properties of the endometrial epithelium in vivo. Materials and Methods: Mouse endometrial epithelial cells were...
متن کاملExperimental Investigation of Ki67, POU5F1, and ZBTB16 Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR
Introduction: Spermatogonial Stem Cells (SSC) are the originators and beginning points of the spermatogenesis process. Moreover, they are considered the only stem cells in the body that could transfer genetic information to the next generation through gametogenesis. This study aimed to investigate the potency and power of SSC under in vitro and in vivo conditions. Materials & Methods: Enzym...
متن کاملCombination of In Vivo Cryptorchid Testis and In Vitro Co- Culture System to Obtain High Purification and Proliferation of Mouse Spermatogonial Stem Cells
Background The present study was designed to evaluate the survival and proliferation of spermatogonial stem cells from cryptorchid mouse testis in co-culture system over a 3 weeks period. MaterialsAndMethods Sertoli and spermatogonial cells were isolated from bilateral cryptorchid mouse model testes. Isolated spermatogonial cells were co-cultured with Sertoli cells in minimal essential medium (...
متن کاملThe Effect of Vitrification and in vitro Culture on the Adenosine Triphosphate Content and Mitochondrial Distribution of Mouse Pre-Implantation Embryos
Background: The mitochondria are an important source of adenosine triphosphate (ATP) production in pre-implantation embryo. Therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and ATP content. Methods: The embryos at 2-PN, 4-cell and blastocyst stages were collected from the oviduct of st...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 48 شماره
صفحات -
تاریخ انتشار 1971